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KMID : 0380120010240060349
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Korean Journal of Ecology
2001 Volume.24 No. 6 p.349 ~ p.352
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Effects of Cadmium on Total Lipid Content and Fatty Acids of the Greater Wax Moth, Galleria mellonella
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Shin, Byung Sik
Choi, Ri Na/Lee, Choong Un
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Abstract
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The beekeeping pest insect Galleria mellonella larvae (greater wax moth) were reared on an artificial diet contaminated indipendently with cadmium chloride (CdCl_2) at different concentrations(1.25, 2.50, 5.00, 10.0 20.0, 40.0 ㎍/g food fresh weight). Results of these studies suggested that Cd exposure of G. mellonella may influence its whole body lipid contents. We decided, therefore, to analyze lipid content of G. mellonella exposed to different concentrations of Cd. Lipid concentrations were measured photometrically by phosphovanillin method. Significant decrease in the total lipid content was found in Cd-contaminated larvae and pupae. In this study, six kinds of lipids were separated, and they were phospholipid, monoglyceride, diglyceride, sterol, fatty acids, triglyceride. And fatty acids were proved to consist of palmitic acid, stearic acid, oleic acid and linolenic acid. Palmitic acid, linoleic acid and oleic acid were found high rate in all groups, but stearic acid was low. In conclusion, lipid contents decreased in Cd-contaminated groups in comparison to the control group.
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